Isolation and Characterisation of Shiga Toxigenic Escherichia Coli Strains From Northern Palestine

Journal Title, Volume, Page:

J Med Microbiol April 2002 vol. 51 no. 4 332-335

Year of Publication:

2002

Link:

http://jmm.sgmjournals.org/content/51/4/332.abstract

Authors:

K. ADWAN

Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine

Current Affiliation:

Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine

[email protected]

N. ABU-HASAN

Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine

T. ESSAWI

Department of Biological Sciences, Bir Zeit University, Palestine

M. BDIR

Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine

Preferred Abstract (Original):

Shiga toxigenic Escherichia coli (STEC) isolates from symptomatic and asymptomatic patients in northern Palestine in 1999 were screened for serotype O157 and characterised for virulence genes by multiplex PCR assay. Of the 176 STEC isolates, 124 (70.5%) were of serotype O157. All these isolates carried the gene for Shiga toxin type 1 (stx₁) and 112 (90.3%) carried stx₂. The intimin encoding gene locus eae was detected in 16 isolates (12.9%) and the enterohaemolysin encoding gene, hlyA, in 18 (14.5%). Statistical analysis showed a significant association between the presence of eaeA and hlyA, either alone or combined with stx₁ and stx₂ genes in O157 isolates from symptomatic infection. ERIC-PCR analysis of DNA from 80 serotype O157 isolates revealed three major clonal populations

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Isolation and Characterisation of Shiga Toxigenic Escherichia Coli Strains From Northern Palestine