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Fast DNA Isolation and PCR Protocols for Detection of Methicillin-Resistant Staphylococci

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Sun, 2014-03-02 13:53 — Kamel Mohammed Dauod Adwan
Journal Title, Volume, Page: 
Folia Microbiologica January 2014, Volume 59, Issue 1, pp 5-8
Year of Publication: 
2014
Link: 
http://link.springer.com/article/10.1007%2Fs12223-013-0259-1
Authors: 
Kamel Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
[email protected]
Preferred Abstract (Original): 

A rapid method that included simple boiling DNA extraction followed by a fast polymerase chain reaction (PCR) cycling protocol designed to detect mecA, which characterizes methicillin-resistant Staphylococcus aureus (MRSA), was performed. Briefly, the PCR cycling protocol consisted of pre-denaturation at 95 °C for 30 s, 30 cycles of denaturation at 94 °C for 2 s, annealing at 52 °C for 5 s, extension for 10 s, and final extension at 72 °C for 1 min. A good level of reliability of the method was verified. The study has shown that the method described here represents a rapid and accurate DNA extraction and PCR-based identification system of MRSA, thus allowing clinicians to make early identification and early implementation of control measures.