The Phytochemical Analysis and Antioxidant Activity Assessment of Orange Peels (Citrus Sinensis) Cultivated in Greece-Crete Indicates a New Commercial Source of Hesperidin

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Journal Title, Volume, Page: 
Biomedical Chromatography Volume 23, Issue 3, pages 239–249
Year of Publication: 
2009
Authors: 
Firas I. Kanaze
Laboratory of Pharmacognosy, Department of Pharmacognosy-Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece
Current Affiliation: 
Department of Pharmacy,Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine
Aikaterini Termentzi
Laboratory of Pharmacognosy, Department of Pharmacognosy-Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece
Chrysi Gabrieli
Laboratory of Pharmacognosy, Department of Pharmacognosy-Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece
Ioannis Niopas
Laboratory of Pharmacology, Department of Pharmacognosy-Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece
Manolis Georgarakis
Department of Pharmaceutical Technology, School of Pharmacy, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece
Eugene Kokkalou
Laboratory of Pharmacognosy, Department of Pharmacognosy-Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece
Preferred Abstract (Original): 

The flavonoid content of several methanolic extract fractions of Navel orange peel (flavedo and albedo of Citrus sinensis) cultivated in Crete (Greece) was first analysed phytochemically and then assessed for its antioxidant activity in vitro. The chemical structures of the constituents fractionated were originally determined by comparing their retention times and the obtained UV spectral data with the available bibliographic data and further verified by detailed LC-DAD-MS (ESI+) analysis. The main flavonoid groups found within the fractions examined were polymethoxylated flavones, O-glycosylated flavones, C-glycosylated flavones, O-glycosylated flavonols, O-glycosylated flavanones and phenolic acids along with their ester derivatives. In addition, the quantitative HPLC analysis confirmed that hesperidin is the major flavonoid glycoside found in the orange peel. Interestingly enough, its quantity at 48 mg/g of dry peel permits the commercial use of orange peel as a source for the production of hesperidin. The antioxidant activity of the orange peel methanolic extract fractions was evaluated by applying two complementary methodologies, DPPH assay and the Co(II)/EDTA-induced luminol chemiluminescence approach. Overall, the results have shown that orange peel methanolic extracts possess moderate antioxidant activity as compared with the activity seen in tests where the corresponding aglycones, diosmetin and hesperetin were assessed in different ratios. Copyright © 2008 John Wiley & Sons, Ltd.

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