Phytochemical Analysis of Anchusa Arvensis Roots

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Journal Title, Volume, Page: 
Planta Medica, Issue 09, Volume 74, p. 1098
Year of Publication: 
2008
Authors: 
FI Kanaze
School of Pharmacy, Department of Pharmaceutics and Drug Control, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
Current Affiliation: 
Department of Pharmacy,Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine
SK Tsermentseli
Organic Chemistry Laboratory, Chemical Engineering Department, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
AN Assimopoulou
Organic Chemistry Laboratory, Chemical Engineering Department, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
N Gianovits-Argyriadou
SkyLab-Med, 26 Amyclon, Chalandri 152 31 Athens, Greece
VP Papageorgiou
Organic Chemistry Laboratory, Chemical Engineering Department, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
Preferred Abstract (Original): 

Organic Chemistry Laboratory of the Chemical Engineering Department at AUTh has a long tradition in Natural Products Chemistry specifically in the isolation, purification and development of quinone secondary metabolites [1–3]. The plant Anchusa arvensis or Lycopsis arvensis belongs to the Boraginaceae family and as many other genera of the same family, biosynthesizes in its roots naphthoquinone pigments of the enantiomeric Alkannins and Shikonins group.

Alkannin, Shikonin and their derivatives are a new class of drugs and exhibit mainly wound-healing, anti-inflammatory and antimicrobial activity. Recent studies have distinguished these compounds as potent antitumor agents. As far as we know, there have not been reported a study of the red pigments biosynthesized in the plant roots of Anchusa arvensis hitherto. Therefore, it is of great interest for our laboratory the isolation and structure identification of hydroxynaphthoquinone pigments of the roots, as well as their accompanying substances, such as lipids, waxes and alkaloids.

HPLC-DAD was applied for the identification of naphthoquinone pigments using standards, while lipids, waxes and alkaloids were identified using GC-MS. Alkannin/Shikonin and their esters acetyl-, β,β-dimethylacryl-, isobutyl- and isovaleryl- ones were determined in the mixture of pigments isolated fromAnchusa arvensis roots.