Purpose: this study aims to approve the folk uses for Ecballium elaterium [(L.) A. Rich.] for treatment of hemorrhoids, varicose veins also for nose bleeding in the West Bank-Palestine. We search about phytochemicals, which improve elasticity of blood vessels. One of the most famous compounds with that effect is phytomelin. Method: a qualitative and quantitative analysis of phytomelin from leaves, fruits, and flowers of Ecballium elaterium (L.) A. Rich (exploding cucumber) in Palestine. After drying the sample for 30 days at 60 °C, phytomelin was extracted using methanol. The methanol extracts of these parts were quantified using reversed phase liquid chromatographic method. Isocratic elution was employed using a mixture of methanol, acetonitrile and a 0.28% (v/v) of acetic acid (5:10:35). The flow rate was 1 ml/min. Results: calibration of the overall analytical procedure gave a linear signal(r>0.998) over a concentration range of 0.1 -0.6 mg/mL of phytomelin. The proposed method was successfully applied for quantitative determination of phytomelin in leaves, fruits, and flowers of Ecballium elaterium (L.) A. Rich Keywords: Exploding cucumber, Ecballium elaterium, HPLC analysis, glycoside, Phytomelin content.