Spectrophotometric Determination of Tiopronin in Pharmaceutical Preparations

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Journal Title, Volume, Page: 
An-Najah University Journal for Research - Natural Sciences - Volume 15, Issue 1, 2001
Year of Publication: 
Maher Abu-Eid
Department of Chemistry, An-Najah National University, Nablus, Palestine
Nidal Zatar
Department of Chemistry, An-Najah National University, Nablus, Palestine
Tamara Kamal
Drug Quality Control Unit, An-Najah National University, Nablus, Palestine
Mohammad Hannoun
Faculty of Pharmacy, An-Najah N. University, Nablus, Palestine
Preferred Abstract (Original): 
Two spectrophotometric methods are described for the determination of tiopronin in pharmaceuticals. They are based on the oxidation-reduction reaction between tiopronin and iron (III), then forming a complex between iron (II) and ferrozine or di-2-pyridyl ketone-2-thiophenoylhydrazone. The produced colored iron (II)-ferrozine complex [system I] absorbs at 562 nm, while the iron (II)-di-2-pyridyl ketone-2-thiophenoylhydrazone complex [system II] absorbs at 656 nm. The effect of different factors such as: pH, reagent concentration, time of reaction, temperature and the tolerance amount of the common excipients have been studied. Applying the optimum working conditions, tiopronin can be determined over the range 0.2-8.6 and 0.5-17.0 ppm and with molar absorptivities of 2.0x10~4 and 1.0x10~4 l mol~-1cm-1 for systems I and II, respectively. Both methods offer high selectivity, sensitivity and accuracy with a relative standard deviation (RSD) of less than 1.1% for five measurements. The proposed methods were applied successfully for the determination of tiopronin in Captimer tablets. Key Words: Spectrophotometry, Tiopronin, Ferrozine, Di-2-pyridyl ketone-2-Thiophenoylhydrazone (DPKTH), Pharmaceutical analysis.
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