Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
Maher A. Abu-Eid
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
Waheed J. Al-Jondi
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
Ahmad I. Hussein
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
Mohammed S. Ali-Shtayeh
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Preferred Abstract (Original):
A new method for isolation and spectrophotometric
determination of plumbagin is presented. Plumbagin was isolated by thin
layer chromatography (TLC) and column chromatography (CC) techniques, as
an orange tinged yellow long crystalline substances. Plumbagin exhibits
two absrop-tion maxima at 410 and 510 nm. Stability of the color, pKa
value, and the effect of pH were studied. Beer's law is obeyed over the
range 0.9-45 ppm. The method is applied to the determination of
plumbagin in roots, stems, and leaves of Plumbago europaea L. plant.