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Emodin, A Naturally Occuring Anthraquinone: Its Isolation and Spectrophotometric Determination in Rumex Cyprius Plant

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Thu, 2015-05-14 12:05 — Mohammed A. A. Hannoun
Journal Title, Volume, Page: 
Spectroscopy Letters, Volume 29, Issue 8 December 1996 , pages 1539 - 1543
Year of Publication: 
1996
Link: 
http://www.tandfonline.com/doi/abs/10.1080/00387019608007144#.VVSEQZP-n_I
Authors: 
Mohammed A. Al-Nuri
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
Nidal A. Za'tar
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
Maher A. Abu-Eid
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
Mohammad A. Hannoun
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
Waheed J. Al-Jondi
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
Ahmad I. Hussein
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
Mohammad S. Ali-Shtayeh
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Preferred Abstract (Original): 
A new method for isolation and specrophotometric determination of emodin is presented. Emodin was isolated by thin layer chromatography (tlc) and column chromatography (cc) techniques, as an orange long crystalline substance. Emodin exhibits two absorption maxima, at 420 and 520 nm. Stability of the color and the effect of pH were studied. Beer's law is obeyed in the range 2-30 ppm.The method is applied to the determination of emodin in roots, stems, and leaves of Rumex cyprius plant.